生物技术通报Issue(7)：82-85,93,5.
烟草ESTs资源的SSR信息分析
Analysis of SSR Information in ESTs Resource of Tobacco (Nicotiana tabacum)
摘要
Abstract
In this research, 242 683 expressed sequence tags (ESTs) were analyzed by software, indicating that 9 339 SSR were found in 211 728 non-redundant tobacco ESTs, and the average distance between SSR was approximately 14.21 kb, accounting for 4.41% of ESTs, including 216 kinds of repeat motifs were mined out. Trimeric repeats, accounting for 50.34%, was dominant in all SSR, followed by dimeric and mono-meric repeats, 23.00% and 16.48%, while the frequency for other repeat type was below 5% each. The most abundant motif was A/T, accounting for 14.68% in all repeat types, followed by AT/TA,AG/TC,AAG/TTC, accounting for 10.49%,9.48%,6.85%, respectively.10 pairs of primers for EST-SSR randomly designed were subjected to PCR for DNAs from six tobacco varieties and one pair of primers showed polymorphisms. The result provided a base for the development and application of EST-SSR markers in tobacco.关键词
烟草/EST-SSR/重复基元Key words
Tobacco EST-SSR Motif分类
农业科技引用本文复制引用
胡重怡,蔡刘体,陈兴江..烟草ESTs资源的SSR信息分析[J].生物技术通报,2009,(7):82-85,93,5.基金项目
贵州省烟草专卖局项目(黔烟科2007-03) （黔烟科2007-03）
