福建农林大学学报（自然科学版）2006，Vol.35Issue(1)：82-86,5.
植酸酶液体发酵条件研究
Optimum conditions for phytase liquid fermentation
汪世华 1杨燕凌 2胡开辉2
作者信息
- 1. 福建农林大学生物农药与化学生物学教育部重点实验室,福建,福州,350002
- 2. 福建农林大学生命科学学院,福建,福州,350002
- 折叠
摘要
Abstract
Optimization of the fermentation medium for maximum phytase production was carried out using a new strain, Trichoderma viride LH374, and the test results were listed as follows: carbon source was 5% glucose, nitrogen source were 3% tryptone and 0.3% NH4NO3, and metallic ion concentration were 0.003% Fe2+ and 0.003% Mn2+. The 500 mL flasks were filled with 50 mL media with pH 6.5 to incubate 72 h, and inoculation amount was 5%. Under optimum condition, Trichoderma viride LH374 was able to yield the maximum phytase activity of 2510 U·mL-1 and the average of 2490 U·mL-1 phytase.关键词
植酸酶/液体发酵/最适条件Key words
phytase/liquid fermentation/optimum condition分类
生物科学引用本文复制引用
汪世华,杨燕凌,胡开辉..植酸酶液体发酵条件研究[J].福建农林大学学报（自然科学版）,2006,35(1):82-86,5.
