解放军医学杂志2009，Vol.34Issue(7)：833-837,5.
质膜微区对土拉弗朗西斯菌LVS感染鼠巨噬细胞的影响
Influence of membrane microdomin on infection process of mouse macrophages by Francisella tularensis LVS
摘要
Abstract
Objective To evaluate the role of membrane microdomain on the entry process of Francisella tularensis LVS infecting mouse macrophages.Methods A shuttle plasmid pFNLTP6 gro-gfp was introduced to E tularensis LVS by dectroporation method.Membrane was stained with FM4-64,and cavcolin-1 was detected with mono-antihody and visualized with Alexa Fluor 594 conjugated goat-antkmouse antibodies.F.tularensis INS infection was analyzed by a fluorescence microscope equipped with a motorized Z-focus.In order to evaluate the depletion of membrane microdomain induced by Francisella entering macrophages,interference with lipid-rich plasma membrane through the depletion of cholesterol was pedormed by treatment with methyl beta cyclodextrin.The cholesterol-binding agent Filipin Ⅲ was used to detect the effect of cholesterol deplction.Results It was shown that the live vaccine strain of E tularensis induced membrane domains ruffling for sucoessful entry into maerophages.Menlbrane microdomain-assodated components such as caveolin1 were incorporated into Francisella containing vesicles when entering the host cell Francisella requires cholesterol-rich membrane domains for entering into macrophages.Conclusion It is suggested that cholesterol-rich membrane microdomains and caveolin-1 play an important role in the early stage of Francisella entering into macrophages.关键词
巨噬细胞/拉弗朗西斯菌/质膜微区/微囊蛋白-1/胆同醇Key words
macrophagcs/Francisella tularensis/membrane microdomaim,cavcolin 1/cholesterol分类
医药卫生引用本文复制引用
潘欣,祁建城,李广渡,贾文凯,瞿曼,赵子夜..质膜微区对土拉弗朗西斯菌LVS感染鼠巨噬细胞的影响[J].解放军医学杂志,2009,34(7):833-837,5.基金项目
Supported by the Foundation for Supporting Returned Overseas Chinese Scholars,State Edueation Ministry(2008 890 2),and by the National Natural Science Foundation of China(30170891) （2008 890 2）
