中国组织工程研究与临床康复2008，Vol.12Issue(42)：8380-8383,4.
小鼠肝细胞胰岛素与表皮生长因子信号转导磷蛋白质组的对比分析
Dynamic phosphoproteomics of insulin and epidermal growth factor signaling in mouse hepatocytes
摘要
Abstract
BACKGROUND: Both epidermal growth factor (EGF) and insulin transfer their signals into cells by two primary signal transduction pathways,including phosphatidylinositol 3-kinase (PI3K) pathway and mitogen activated protein kinases (MAPK) pathway.But they have different physiological functions.OBJECTIVE: To comparatively assay the dynamic behaviors of phosphoproteomes between EGF and insulin signal transductions in mouse hepatocytes and find key signal proteins.DESIGN,TIME AND SETTING: Randomized grouping controlled observation experiment was performed in the laboratory of Molecular Biology,Luzhou Medical College between July 2005 and April 2006.MATERIALS: Hepatocytes were from Kunming mice of closed population.METHODS: The primarily cultured mouse hepatocytes were labeled with 32p isotope and then randomly divided into three groups: control,EGF-stimulated (received 10 μg/L EGF),and insulin-stimulated (received 100 nmol/L insulin) groups.MAIN OUTCOME MEASURES: After mouse hepatocytes were treated with EGF and insulin for 0,5,20,60 and 120 minutes,the dynamic behaviors of phosphoproteomes(I.e,phosphorylated level) between EGF and insulin signal transductions were comparatively analyzed by two-dimensional electrophoresis method.RESULTS: The categories of all phosphorylated proteins between EGF and insulin-stimulated phosphoproteomes had no apparent difference.The dynamic behaviors of phosphoproteomes of most proteins during EGF signal transduction are parallel with those during insulin stimulation,except the dynamic behaviors of 4 proteins are different significantly.CONCLUSION: Aforementioned 4 phosphorylated proteins were most probably the key members that could distinguish between two signal transduction pathways ornetworks,and determined their major physiological functions respectively.关键词
胰岛素/表皮生长因子/磷酸化蛋白质/信号转导系统/动力学行为分类
医药卫生引用本文复制引用
刘友平,姚富丽,丁慧荣,李洪..小鼠肝细胞胰岛素与表皮生长因子信号转导磷蛋白质组的对比分析[J].中国组织工程研究与临床康复,2008,12(42):8380-8383,4.基金项目
"细胞信号转导的复杂性与计算机模拟":国家自然科学基金资(70071040) （70071040）
Supported by: the National Natural Science Foundation of China,No.70071040 （）
