中德临床肿瘤学杂志（英文版）2005，Vol.4Issue(2)：83-85,3.
化疗增敏剂影响P-糖蛋白依赖多药耐药性肿瘤细胞99Tcm-MIBI摄取的实验研究
Using 99mTc-MIBI to Evaluate the Effects of Chemosensitizer on P-glycoprotein in Multidrug-resistant Carcinoma Cells
张振蔚 1张雪梅 1吴华 1赵明 1鲜于志群 1周健 1赖世英1
作者信息
- 1. 武汉华中科技大学同济医学院附属同济医院核医学科,430030
- 折叠
摘要
Abstract
Objective: To establish a method to evaluate the effects of chemosensitizer on P-glycoprotein using 99mTc-MIBI, and observe the changes of 99mTc-MIBI uptake kinetics and P-glycoprotein levels after using verapamil in MDR human breast cells MCF-7/Adr. Methods: MDR breast carcinoma cells, MCF7/Adr, were incubated and different protocols were performed. Protocol Ⅰ: a chemosensitizer, verapamil (10 μmol/L), was added into cell culture medium, while in control group, the same volume of DMEM was given. Cells were harvested after 2 h incubation with 99mTc-MIBI. Protocol Ⅱ: Verapamil (10 μmol/L)was added into cell culture medium and incubated for 20 min, 40 min, 60 min, 80 min, 8 h, 24 h, 48 h and 72 h respectively. Cells were harvested after 2 h incubation with 99mTc-MIBI. The radioactivity of the cells was measured and P-glycoprotein expression levels were determined with immunohistochemical stain. Results: Protocol Ⅰ: After 2h incubation with verapamil the cellular uptake of 99mTc-MIBI was remarkably higher than control group (t=2.33, P＜0.05), but there was no difference in P-glycoprotein expression levels between two groups (P＞0.05). Protocol Ⅱ: In verapamil group, 99mTc-MIBI uptake was increased with incubation time prolonging (F=58.2, P＜0.05). When verapamil incubation time surpassed 8h the 99mTc-MIBI uptake negatively correlated to the P-glycoprotein expression levels (r=-0.73, P＜0.01).However, when incubation time was less than 80 min, there was no correlation between 99mTc-MIBI accumulation and P-glycoprotein levels (r=0.16, P＞0.05). Conclusion: 99mTc-MIBI may be used to evaluate the qualitative as well as quantitative change of P-glycoprotein expression levels induced by the chemosensitizer, verapamil.关键词
多药耐药性/化疗增敏剂/乳腺癌/P-糖蛋白/MIBIKey words
multidrug resistance/chemosensitizer/breast tumor/P-glycoprotein/99mTc-MIBI引用本文复制引用
张振蔚,张雪梅,吴华,赵明,鲜于志群,周健,赖世英..化疗增敏剂影响P-糖蛋白依赖多药耐药性肿瘤细胞99Tcm-MIBI摄取的实验研究[J].中德临床肿瘤学杂志（英文版）,2005,4(2):83-85,3.
