首页|期刊导航|中华医学杂志（英文版）|Preliminary separation of the growth factors in platelet-rich plasma: effects on the proliferation of human marrow-derived mesenchymal stem cells
中华医学杂志（英文版）2009，Vol.122Issue(1)：83-87,5.DOI:10.3760/cma.j.issn.0366-6999.2009.01.015
Preliminary separation of the growth factors in platelet-rich plasma: effects on the proliferation of human marrow-derived mesenchymal stem cells
Preliminary separation of the growth factors in platelet-rich plasma: effects on the proliferation of human marrow-derived mesenchymal stem cells
摘要
Abstract
Background Platelet-rich plasma (PRP) as a storage vehicle of growth factors has been successfully used in clinical applications, but in most cases the platelets were autologous. However, the large volume of blood withdrawn has detrimental effects on patients with anemia or poor general health. To overcome these limitations, this study was designed to separate the growth factors in homologous platelet-rich plasma. Methods The gel chromatography with Superdex-75 column was applied to separate PRP supernatants into 4 major fractions. Then the four fractions were vacuumed freeze-dried and re-dissolved in phosphate buffered saline. Proteins concentrations in PRP and in four fractions were detected by bicinchoninic acid protein assay; platelet derived growth factor-AB (PDGF-AB) and transforming growth factor 131 (TGF-β1) levels were determined by sandwich enzyme-linked immunosorbent assays. The effects of fractions on the proliferation of human marrow-derived mesenchymal stem cells (MSCs) were determined by 3-(4, 5- dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Results PRP supernatants were separated into four major fractions by gel chromatography. The proteins recovery was 96.72%. Of the four fractions, fraction B contained the highest TGF-β1 and PDGF-AB levels, and the highest proteins concentrations. Cell proliferation curves of MSC demonstrated that fraction B and C induced a remarkable increase of MTT values compared to the untreated culture (P <0.05), and the effects of fraction B and C showed no significant difference compared to the PRP group (P >0.05). Fraction A and D showed no significant difference to the negative control group (P >0.05). Conclusions The growth factors in PRP supernatants could be preliminarily separated into four fractions by gel chromatography, and the freeze-drying fractions retained the biological activity of growth factors. The growth factors were mostly presented in fraction B and C, and they promoted cell proliferation effectively.关键词
platelet-rich plasma/gel chromatography/mesenchymal stem cellKey words
platelet-rich plasma/gel chromatography/mesenchymal stem cell分类
医药卫生引用本文复制引用
HUANG Qian,WANG Yun-dan,WU Tao,JIANG Shan,HU Yan-ling,PEI Guo-xian..Preliminary separation of the growth factors in platelet-rich plasma: effects on the proliferation of human marrow-derived mesenchymal stem cells[J].中华医学杂志（英文版）,2009,122(1):83-87,5.基金项目
This work was supported by a grant from National Natural Science Foundation of China (No. 30600643). （No. 30600643）
