中国肺癌杂志2001，Vol.4Issue(2)：83-87,5.
反义VEGF基因及内皮抑素基因转染对人巨细胞肺癌生物学行为的影响
Inhibitory effect of antisense VEGF121 and endostatin genes transfection on tumor growth and metastasis of human giant cell lung cancer
吴晓 1郑杰 1朱建健 1付坚 1马春树 1由江峰 1崔湘琳 1王洁良 1方伟岗 1周爱儒 1汤健 1吴秉铨1
作者信息
摘要
Abstract
bjective To explore the co-operative inhibitory effect ofantisense VEGF gene and endostatin gene transfection on tumor angiogenesis, tumor growth and metastasis of lung cancer. Methods Antisense VEGF121 cDNA was transfected into PG cells(PG-AS-VEGF) by lipofectin. After PG-AS-VEGF cells were xenografted to nude mice, PsectagA-endostatin gene was transfected into nude mice by electric pulse mediation. The MVDs in tumors and tumor biological characteristics were observed. Results ①The MVD in PG-AS-VEGF tumor in nude mice was significantly lower than that in PG-vector tumor (PG-AS-VEGF and PG-vector: 40.67±9.35 and 58.34±10.52, respectively) in nude mice. ②There was no significant difference between the PG-vector tumor and PG-AS-VEGF tumor in early stage of the tumor growth in vivo. However, PG-AS-VEGF tumor grew significantly more slowly than PG-vector tumor after 18 days (P＜0.05). ③PG-AS-VEGF tumor could lead to regional and／or distant lymph node metastases (16.7％, 2／12）, which was much more infrequent than that in PG-vector group (50％, 6／12). ④ PG-AS-VEGF tumor growth was remarkably inhibited by endostatin gene transfected at site of the tumor inoculation as compared with the control group in nude mice (P＜0.05). ⑤The PG-AS-VEGF tumors transfected with the endostatin gene at site of the tumor inoculation(AST) could also produce much lower regional and／or distant lymph node metastases rate (12.5％, 1／8） than that in the PG-AS-VEGF tumor transfected with the PsectagA vector (ASP)(75％, 6／8). Conclusion Endostatin gene transfection could cooperatively inhibit the growth and spontaneous lymph node metastasis of antisense VEGF gene transfected PG cells in nude mice.关键词
肺肿瘤/血管内皮生长因子/内皮抑素/基因转染分类
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吴晓,郑杰,朱建健,付坚,马春树,由江峰,崔湘琳,王洁良,方伟岗,周爱儒,汤健,吴秉铨..反义VEGF基因及内皮抑素基因转染对人巨细胞肺癌生物学行为的影响[J].中国肺癌杂志,2001,4(2):83-87,5.
