中国临床康复2004，Vol.8Issue(36)：8406-8407,2.
缬沙坦对自发性高血压大鼠心肌Bcl-2和Bax蛋白表达的影响
Effect of valsartan on the expressions of Bcl-2 and Bax proteins in myocardium of spontaneously hypertensive rats
刘巍 1李为民 1孙宁玲2
作者信息
- 1. 哈尔滨医科大学第一临床医学院心内科,黑龙江省,哈尔滨市,150001
- 2. 北京大学人民医院心内科,北京市,100044
- 折叠
摘要
Abstract
BACKGROUND: An increase in cardiac work-load during hypertension promotes an adaptive response consisting of cardiomyocytes hypertro phy. Although this response is initially compensatory, continuing cardiac overload leads to a decreased global cardiac function and subsequently to heart failure, and among the mechanisms involved, that contributing to the progressive loss of cardiomyocytes has attracted more and more attention.OBJECTIVE: To explore the changes of the expressions of apoptosis regulating oncogene Bcl-2 and Bax in myocardial tissue during genetic hypertension and left ventricular hypertrophy, and evaluate the effect of valsartan, the antagonist to angiotensin Ⅱ type 1 receptor(AT1R) on the ratio of the Bcl-2/Bax.DESIGN: A randomized controlled study.SETTINGS: Cardiological department in a medical college of university anda university hospital.MATERIALS: The trial was completed in the Central Laboratory, People's Hospital of Peking University. Thirty spontaneously hypertensive rats(SHR)of 8 weeks old were divided into valsartan group and non-treated group according to the method of random number table, and another 15 Wistar rats of 8-week old were taken as the controls.INTERVENTIONS: All the rats were fed for 8 weeks, the left ventricular tissues were excised and weighed for later use. Part of the myocardial tissues were fixed in 100 mL/L buffered formalin for 6 hour, and were embedded by routine paraffin wax for morphological study. The cardiac index was calculated by cardiac mass/body mass. The expressions of apoptosis regulating Bcl-2 and Bax proteins were detected by immunohistochemistry and Western blot.MAIN OUTCOME MEASURES: Expressions of Bcl-2 and Bax protein as scanning band absorbance in Western blot, the ratio of Bcl-2/Bax.RESULTS: There was high expression of Bax protein in the myocardium of SHR, after valsartan treatment for 8 weeks, the expression of Bax protein in the myocardiun of SHR was significantly reduced to the level near to that in the control group. The expressions of Bcl-2 protein in the valsartan group and control group were insignificantly different from that in the non-treated group( P ＞ 0.05) . The ratios of bax/bcl-2 in the valsartan group(6.71± 1.10) and control group(5.86 ±0. 70) were obviously higher than that in the non-treated group(0. 63 ±0.23) ( P ＜ 0.05).CONCLUSION: Cardiac apoptosis is one of the possible mechanisms for compensatory myocardial hypertrophy developing into heart failure. Early valsartan treatment on hypertension has not only the effect of depression, but also can effectively inhibit myocardial apoptosis.关键词
心肌病,肥厚性/基因表达/细胞凋亡/心肌/病理学分类
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刘巍,李为民,孙宁玲..缬沙坦对自发性高血压大鼠心肌Bcl-2和Bax蛋白表达的影响[J].中国临床康复,2004,8(36):8406-8407,2.
