中国临床康复2004，Vol.8Issue(36)：8418-8420,3.
针刺对肥胖大鼠纹状体组织一氧化氮含量和一氧化氮合酶活性的作用
Effects of acupuncture on contents of nitrogen monoxide and nitrogen monoxide synthase in the striatum of obese rats
摘要
Abstract
BACKGROUND: It is known that the mechanism of acupuncture in treating obesity comes up with the neural and neurohumoral regulations. Striatum participates in regulating and controlling food taking centrum, plant nerve centrum and animal heat centrum of hypothalamus. What is the regulating effect of acupuncture on nitric oxide (NO) content and nitric oxide synthase (NOS) activity in striatum tissues?OBJECTIVE: To study the effects of acupuncture on the content of NO and NOS in the striatum tissue of obese rats, and further investigate the mechanism of acupuncture effect on reducing body mass in central nervous system.DESIGN: Self-controlled and mutual controlled experiment.SETTING: Acupuncture Institute of Second Clinical Medical College, Nanjing University of Traditional Chinese Medicine, and Department of Reproduction Medicine, Nanjing College for Population Programmed Management.PARTICIPANTS: This experiment was carried out in the Acupuncture Institute of Second Clinical Medical College. One-month old male SD rats just in ablactation, weighing 50 to 70 g, were provided by the Animal Experiment Center of General Hospital of Nanjing Military Area Command , and the animals were in the clean grade.INTERVENTIONS: Rats fed with ordinary diet were in the normal group. Experimental obese rats models were randomly divided as control group and acupuncture group, 6 in each. Rats in the acupuncture group were given acupuncture treatment for 14 days, and rats in the normal and control groups were put into rat fixation-machine for 15 minutes everyday, lasting for 14 days. Body mass, body lipid and contents of NO and NOS in striatum tissues of the obese rats were observed with nervous biochemical technology before and after acupuncture respectively.MAIN OUTCOME MEASURES: ① Effect of acupuncture on obesity index, fat contents of pericardium, kidney and epididymis of obese rats. ②Effect of acupuncture on NO and NOS contents in striatum tissue of obese rats. ③ Correlation of NO content and NOS activity with body mass and Lee's index in striatum tissue of obese rats.RESULTS: The body mass and body fat content of the obese rats were all significantly higher than those in the normal rats( t = 8.86, 9.99, 8.31, P ＜ 0.01); NO content and NOS activity in the striatum tissue of the obese rats were all markedly higher than those in the normal rats( t =5.48, 4.37,P ＜ 0.01) . NO content and NOS activity were highly negatively correlated with body mass and Lee' s index(r= -0.78, -0.71, P ＜ 0.01) .Acupuncture significantly increased the NO content and NOS activity in the striatum tissue of the obese rats as compared with those before acupuncture(t=3.74, 3.48, P ＜0.01).CONCLUSION: The abnormal levels of NO and NOS in striatum tissue might be an important factor to cause simple obesity. The good regulation of acupuncture on the levels of NO and NOS in striatum tissue might be one of the central nervous mechanisms of reducing body mass in obese organisms.关键词
肥胖症/病理生理学/纹状体/针灸效应/体重/小鼠,肥胖/一氧化氮/代谢/一氧化氮合酶/代谢/针刺疗法分类
医药卫生引用本文复制引用
刘志诚,孙凤岷,袁锦虹,姜军作,吕雅妮,衣运玲..针刺对肥胖大鼠纹状体组织一氧化氮含量和一氧化氮合酶活性的作用[J].中国临床康复,2004,8(36):8418-8420,3.基金项目
国家自然科学基金资助项目(39970923) （39970923）
