眼科新进展2001，Vol.21Issue(2)：84-85,2.
一氧化氮和氧自由基诱导视网膜神经节细胞凋亡
Apoptosis in cultured rat retinal ganglion cells induced by nitric oxide and oxygen free radical
张俊华 1金威尔 1林颖 1张国安2
作者信息
- 1. 350004 福州市，福建省人民医院眼科
- 2. 解放军第476医院临床免疫研究室
- 折叠
摘要
Abstract
Objective To study the apoptosis in cultured rat retinal ganglioncells(RGCs) induced by nitric oxide (NO) and oxygen free radical (O2-),and to explore the relationship between NO and O2-.Methods The SNP (the provider of NO) and XO/X (provider of O2-) were evaluated for their damage to RGCs. Damage was confirmed by cell apoptosis rate and percentage of DNA fragmentation.Results (1) Compared with control group ,cell apoptosis rate and percentage of DNA fragmention caused by SNP increased significantly (P ＜ 0.01) and was related to the concentration of SNP. The apoptosis in RGCs caused by SND can be inhibited evidently after oxygen free radical dismutase(SOD)was added.(2) The results of O2- damage to RGCs were similar to those of SNP.More serious damage was caused by using NO and O2- together than by using NO or O2- separately (P ＜ 0.01).Conclusion Both NO and O2- can induce the apoptosis in cultured RGCs,and there is cooperation between them.关键词
一氧化氮/氧自由基/视网膜神经节细胞/凋亡Key words
nitric oxide/oxygen free radical/retinal ganglioncell/apoptosis分类
医药卫生引用本文复制引用
张俊华,金威尔,林颖,张国安..一氧化氮和氧自由基诱导视网膜神经节细胞凋亡[J].眼科新进展,2001,21(2):84-85,2.
