中风与神经疾病杂志2001，Vol.18Issue(2)：84-86,3.
胶质细胞源性神经营养因子在大鼠
急性痫性发作中的表达
Time course expression of GDNF protein in rat hippocampus after intrahippocampal kainic acid injection induced acute seizure
张贺 1肖波 2王蓉2
作者信息
- 1. 中南大学湘雅二院神经科,
- 2. 中南大学湘雅一院神经科,
- 折叠
摘要
Abstract
Objective To examine the expression of glial cell line-derived neurotrophic factor（GDNF）protein in hippocampus of acute epileptic SD rats. Method Forty Sprague-Dawley rats were randomly divided into two groups: normal control (Group NS) and kainic acid-treated (Group KA). The models were induced by unilateral intrahippocampal injection of KA(0.6μg/0.3μl). GDNF were examined with immunocolloidal-gold-silver staining (IGSS) dynamically at post-injection hours 3, 6 and 24; and post-injection days 4 and 7 in both groups. The density of IGSS staining were analyzed using an imaging analysis system. Results In group NS, GDNF immunoreactivity was very weak, and remained at the normal level at each time point. In group KA, GDNF protein began to increase bilaterally in the dentate gyri and hilar area within 3h after KA injection, reached its maximum level at 6h, then decreased at 24h and PID 4, these were higher than the corresponding control（P<0.05）, and returned to the control level by PID 7. No significant difference was found in GDNF between bilateral dentate gyri at each time point. Conclusion GDNF can be increasingly expressed in the dentate gyri and hilar areas after KA-induced seizures, and may protect the dentate granule cells from KA excitatory toxicity.关键词
胶质细胞源性神经营养因子/红藻氨酸/痫性发作/大鼠/海马分类
医药卫生引用本文复制引用
张贺,肖波,王蓉..胶质细胞源性神经营养因子在大鼠
急性痫性发作中的表达[J].中风与神经疾病杂志,2001,18(2):84-86,3.
