临床骨科杂志2001，Vol.4Issue(2)：84-88,5.
成人间充质干细胞体外成骨的初步研究
A preliminary study of in vitro osteogenesis of culture-expanded,bone marrow-derived adult human mesenchymal stem cells
朱六龙 1孔荣 1孙自敏 2尚希福 1童元 1方诗元 1汪健 2翟志敏 2吴竞生2
作者信息
- 1. 安徽省立医院骨科,
- 2. 安徽省立医院中心实验室,
- 折叠
摘要
Abstract
Objective To establish a method of isolating and culturing adult human bone marrow-derived mesenchymal stem cells(MSCs) and to investigate their ex vivo osteogenic potential. Methods Adult human MSCs derived from marrow were isolated from the Percoll fraction of mononuclear cells, cultured in Dulbecco's Modified Eagle's Medium with low glucose containing 10% fetal bovine serum, and proliferated through a process of subculturing. For in vitro osteogenic differentiation,adult hMSCs from the fifth passage were grown in the presence of osteogenic supplements (100 nmol/L dexamethasone, 10 mmol/L glycerophosphate,and 50 μmol/L vitamin C). On days 4 and 12 of culture, flow cytometric analysis was performed to examine the expression of cell surface molecules on adult hMSCs and samples were assayed for alkaline phosphatase histochemistry and for mineral deposition by Von Kossa staining respectively. Results Adult human MSCs were a homogenous population of adherent bone marrow cells with a distinct cell surface protein expression and morphology. Passaged adult hMSCs responded to the osteogenic supplements by forming interconnected nodular aggregates on the surface of the dish, increasing A Pase-postive cells, and depositing a calcified matrix. Conclusion The isolation and culture conditions established for adult hMSCs may select a distinct population of bone marrow-derived adherent cells. Adult human MSCs possess osteogenic potential in vitro.关键词
骨髓/间充质干细胞/骨生成/成年人分类
医药卫生引用本文复制引用
朱六龙,孔荣,孙自敏,尚希福,童元,方诗元,汪健,翟志敏,吴竞生..成人间充质干细胞体外成骨的初步研究[J].临床骨科杂志,2001,4(2):84-88,5.
