中国人兽共患病学报2007，Vol.23Issue(9)：855-860,6.
重组钩端螺旋体病DNA疫苗pVAX1/LipL21-LipL32诱导免疫应答反应的研究
The immune responses induced by a recombinant DNA vaccine for leptospirosis:pVAX1/LipL21-LipL32
摘要
Abstract
Two highly conserved and with abundant quantity of lipoproteins in outer membrane of pathogenic species,but not in saprophytic species of leptospira ,LipL32 and LipL21,were selected to construct the fusion gene DNA vaccine pVAX1/LipL21-LipL32,and its ability to induce immune responses in BALB/c mice inoculated with this recombinant DNA vaccine was investigated in the present study.Expression of the fusion-protein LiPL21-LiPL32 was demonstrated in HEK293 cells following transfection with the fusion gene DNA vaccine and the immune responses induced after intramuscular inoculation with this DNA vaccine in BALB/c mice was then evaluated by microscopic agglutination test (MAT),meanwhile the ELISA assay was used to detect the cytokines induced.It was demonstrated that significant level of specific antibodies agglutinating antigens of Leptospira interrogans could be detected by MAT after DNA vaccine inoculation.The production of cytokines IL-10 and TNF-β in mice inoculated with DNA vaccine pVAX1/LipL21-LipL32 was significantly increased in comparison with that of the group inoculated with pVAX1 alone.These results indicate that the recombinant DNA vaccine pVAX1/LipL21-LipL32 may be of potential value to design and develop new generation of vaccines against leptospirosis.关键词
钩端螺旋体病/LipL21/LipL32/DNA疫苗Key words
leptospirosis/LipL21/LipL32/DNA vaccine分类
医药卫生引用本文复制引用
冯春燕,李擎天,董珂,杨宏亮,胡宝瑜,郭晓奎..重组钩端螺旋体病DNA疫苗pVAX1/LipL21-LipL32诱导免疫应答反应的研究[J].中国人兽共患病学报,2007,23(9):855-860,6.基金项目
This work was supported by the National Natural Science Foundation of China (No.30370071 & 30670102),the National High Technology Research and Development Program of China (No.2006AA02Z1760) and Shanghai Leading Academic Discipline Project (No.T0206) （No.30370071 & 30670102）
