首页|期刊导航|中国农业科学（英文版）|Isolation,Identification and Tissue Expression Profile Analysis of One Novel Differentially Expressed Sequence Tag in the Longissimus dorsi Muscle from Meishan,Meishan × Large White Hybrid and Large White Pigs
中国农业科学（英文版）2004，Vol.3Issue(11)：856-861,6.
Isolation,Identification and Tissue Expression Profile Analysis of One Novel Differentially Expressed Sequence Tag in the Longissimus dorsi Muscle from Meishan,Meishan × Large White Hybrid and Large White Pigs
Isolation,Identification and Tissue Expression Profile Analysis of One Novel Differentially Expressed Sequence Tag in the Longissimus dorsi Muscle from Meishan,Meishan × Large White Hybrid and Large White Pigs
摘要
Abstract
In order to detect the molecular mechanism of heterosis in pigs, the mRNA differential display technique was performed to investigate the differences of gene expression in the Longissimus dorsi tissue from Meishan, Meishan × Large White hybrid and Large White pigs with nine 3'-end anchored primers in combination with ten 5'-end arbitrary primers and nearly 3 000 reproducible bands were examined. One novel expressed sequence tag (EST4,GenBank accession number: AY553914) that was differentially expressed in Meishan,Meishan × Large White hybrid and Large White pigs was isolated from the Longissimus dorsi muscle tissue and identified through semi-quantitative RT-PCR. BLAST analysis revealed that the 350 bp long EST (EST4) was not homologous to any of the known porcine genes.Tissue expression profile analyses showed that the EST4 was expressed in most of tissues.关键词
mRNA differential display/Semi-quantitative RT-PCR/Tissue expression profile analysisKey words
mRNA differential display/Semi-quantitative RT-PCR/Tissue expression profile analysis引用本文复制引用
LIU Yong-gang,XIONG Yuan-zhu,DENG Chang-yan..Isolation,Identification and Tissue Expression Profile Analysis of One Novel Differentially Expressed Sequence Tag in the Longissimus dorsi Muscle from Meishan,Meishan × Large White Hybrid and Large White Pigs[J].中国农业科学（英文版）,2004,3(11):856-861,6.基金项目
The authors wish to express thanks to Dr.Jiang Xunping for his encouragement and advice. This work was supported by grants from the National "973" Project of China (G2000016105). （G2000016105）
