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中国农业科学（英文版）2007，Vol.6Issue(7)：857-862,6.
Detection of Staphylococcus aureus in Dairy Products by Polymerase Chain Reaction Assay
Detection of Staphylococcus aureus in Dairy Products by Polymerase Chain Reaction Assay
摘要
Abstract
A polymerase chain reaction (PCR) assay was employed for direct detection of Staphylococcus aureus without enrichment in dairy products. A solvent extraction procedure was successfully modified for the extraction of Staphylococcus aureus DNA from artificially contaminated whole milk, skim milk, and cheese. A primer targeting the thermostable nuclease gene (nuc) was used in the PCR. A DNA fragment of 279 bp was amplified. The PCR product was confirmed by DNA sequencing. In this study, the PCR, GB- 4789.10-94, Perifilm RSA.Count Plate, and Baird-Parker + RPF Agar were compared.The sensitivity of the PCR was 10 CFU mL-1 of whole milk, skim milk, and 55 CFU g-1 of cheese. The developed methodology allowed for detection of Staphylococcus aureus in dairy products in less than 6 h. The time taken for the development of this PCR assay was 12-24 h, less than the time taken by the general PCR assay using the enrichment method, and the coincidence rate of this developed PCR was 94.3%, the sensitivity was 100%. It was a rapid, sensitive, and effective method for PCR to detect Staphylococcus aureus in milk and milk products.关键词
PCR/detection/dairy products/Staphylococcus aureusKey words
PCR/detection/dairy products/Staphylococcus aureus分类
农业科技引用本文复制引用
YANG Yang,SU Xu-dong,YUAN Yao-wu,KANG Chun-yu,LI Ying-jun,ZHANG wei,ZHONG Xiao-ying..Detection of Staphylococcus aureus in Dairy Products by Polymerase Chain Reaction Assay[J].中国农业科学（英文版）,2007,6(7):857-862,6.基金项目
The study was supported by the Scientific Research Program of the Agricultural University of Hebei, China(2005-01). （2005-01）
