首页|期刊导航|中华医学杂志（英文版）|The role of calcineurin in the lung fibroblasts proliferation and collagen synthesis induced by basic fibroblast growth factor
中华医学杂志（英文版）2003，Vol.116Issue(6)：857-862,6.
The role of calcineurin in the lung fibroblasts proliferation and collagen synthesis induced by basic fibroblast growth factor
The role of calcineurin in the lung fibroblasts proliferation and collagen synthesis induced by basic fibroblast growth factor
摘要
Abstract
Objective To investigate the role of calcineurin (CaN) in the lung fibroblast proliferation and collagen synthesis induced by basic fibroblast growth factor (bFGF).Methods We used Western blot and immunohistochemical methods for investigating the content and distribution of calcineurin in the lung tissue. Calcineurin activity in different tissues was measured using 32P-labelled substrate. In the primary culture of lung fibroblasts, 3H-thymidine (3H-TdR) and 3H-proline incorporation methods were used to study the effect of cyclosporin A(CsA), an inhibitor of calcineurin, on the lung fibroblast DNA and collagen synthesis stimulated by bFGF. Results We found that calcineurin was expressed in lung tissue and has phosphatase activity (7.1±2.0 pmol Pi/mg pr/min). CsA(10-8-10-6mol/L) inhibited lung fibroblast，3H-TdR incorporation induced by bFGF in a dose-dependent manner, with the inhibitory rates by20%, 46% and 66%(P<0.01). CsA(10-7-10-6mol/L) inhibited 3H-proline incorporation in lung fibroblasts stimulated by bFGF, with the inhibitory rates by 21% and 37%(P<0.01). In a culture medium, CsA (10-8-10-6mol/L) inhibited 3H-proline secretion induced by bFGF in a dose-dependent manner, with the inhibitory rates by 19%,29%(P<0.05) and 56% (P<0.01). CsA (10-7mol/L) could inhibit calcineurin activity by 44% in lung fibroblasts(P<0.01). Conclusions Calcineurin is expressed in lung tissue and has phosphatase activity. It is involved in the bFGF stimulated lung fibroblast DNA and collagen synthesis.关键词
calcineurin/lung fibroblast/collagen/proliferation/basic fibroblast growth factorKey words
calcineurin/lung fibroblast/collagen/proliferation/basic fibroblast growth factor分类
医药卫生引用本文复制引用
陈亚红,赵鸣武,符民桂,姚婉贞,唐朝枢..The role of calcineurin in the lung fibroblasts proliferation and collagen synthesis induced by basic fibroblast growth factor[J].中华医学杂志（英文版）,2003,116(6):857-862,6.基金项目
The study was supported by the National Natural Science Foundation of China (No.39730220). （No.39730220）
