首页|期刊导航|高技术通讯（英文版）|Modification of the rib operon derived from Bacillus subtilis and its expression in Escherichia coli
高技术通讯（英文版）2007，Vol.13Issue(1)：85-90,6.
Modification of the rib operon derived from Bacillus subtilis and its expression in Escherichia coli
Modification of the rib operon derived from Bacillus subtilis and its expression in Escherichia coli
摘要
Abstract
A riboflavin operon(rib operon)derived from Bacillus subtilis 368 was modified on structure and the resulting operons were expressed in various strains of Escherichia coli. The results showed that the optimization of the rib operon and the host strain used for expression are two main factors affecting the riboflavin production. Replacing the promoter l and rfn box of the rib operon with a strong constructive promoter spo l drastically increased the expression of the rib genes. When E. Coli JMl09 was used as the host strain, the highest riboflavin production reached 95.3μg/mL(about eight times higher than that 0f the unmodified rib operon). In addition, when tetracycline(20 μg/mL)was used as the selective pressure, compared with the ampicillin resistant transformants, a higher riboflavin yield Was obtained in tetracycline resistant host strain.关键词
riboflavin/operon/Escherichia coli/Bacillus subtilisKey words
riboflavin/operon/Escherichia coli/Bacillus subtilis分类
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Zhang Huitu,Meng Kun,Wang Yaru,Luo Huiying,Yuan Tiezheng,Yang Peilong,Bai Yingguo,Yao Bin,Fan Yunliu..Modification of the rib operon derived from Bacillus subtilis and its expression in Escherichia coli[J].高技术通讯（英文版）,2007,13(1):85-90,6.基金项目
Supported by the High Technology Research and Development Progamme of China(No.2001AA214041 and 2003AA214030). （No.2001AA214041 and 2003AA214030）
