中德临床肿瘤学杂志（英文版）2005，Vol.4Issue(2)：86-88,3.
建立一种新的多药耐药的诱导方法
Establishment of a mdr1 Multidrug Resistant Model of Orthotopic Transplantation of Liver Carcinoma on Nude Mice
摘要
Abstract
Objective: To develop a new method of inducing mdr1 multidrug resistance by establishing a nude mice model of orthotopic transplantation of liver carcinoma by sporadic abdominal chemotherapy at intervals. Methods: Hepatocellular carcinoma HepG2 cell was cultured and injected subcutaneously to form the tumor-supplying mice. The tumor bits from the tumor-supplying mice were implanted under the envelope of the mice liver and induced by abdominal chemotherapy with Pharmorubicin. Physical examination, ultrasonography, spiral CT and operative inspection were used to examine tumor progression. RT-PCR and immunohistochemistry were adopted to detect the expression of mdr1-mRNA and its encoded protein P-gp protein (P-gp). Results: There was no operative dead, the rate of implanting tumor successfully was 88% (22/25), the rate of implanting secondly successfully was 100% (3/3), and the rate of inducing successfully was 80% (16/20). The expression of mdr1-mRNA and the P-gp in the inducing group was 23 folds and 13 folds in the control group respectively. Conclusion: We have established an in uivo model of mdr using nude mice transplanted with orthotopic liver neoplasm coupled to chemotherapy.关键词
肝细胞癌/多药耐药基因/裸小鼠/动物模型Key words
liver neoplasms/genes, MDR/mice, nude/disease models, animal引用本文复制引用
韩宇,陈孝平..建立一种新的多药耐药的诱导方法[J].中德临床肿瘤学杂志（英文版）,2005,4(2):86-88,3.基金项目
This project was supported by a grant from the Medical Science and Technique Foundation of Ministry of Health of China(No. Wkz-2000-1-15). （No. Wkz-2000-1-15）
