生物技术通报Issue(7)：86-89,4.
蒙古冰草Actin基因片段的克隆及序列分析
Isolation and Characterization of Actin Gene Fragment from Agropyron mongolicum Keng
摘要
Abstract
In this study,Actin gene homology fragment from Mongo1ian wheatgrass(Agropyron mongolicum Keng)was isolated using homology-based method, so that it can be used as internal standard when study other genes' expression and regulation in Mongo1ian wheatgrass.Two pairs of primers A4 and A5 was designed according to the amino acid conserved regions of the cloned actin gene of Gramineae plant wheat, and two partials gene sequences were obtained by reverse transcription polymerase chain reaction(RT-PCR),the product of which was named as MwACT.The sequences was 962 bp after merging two partials gene repeat sequences,which encoded 237 amino acids. Homology comparison with Actin gene sequences in other plants was over 79%,which was 94% identical to wheat and barley.Over 90%amino acid sequence homology was showed with other plants.关键词
蒙古冰草/Actin基因/克隆/序列分析Key words
Agropyron mongolicum Keng Actin gene Clone Sequence analysis分类
农业科技引用本文复制引用
赵彦,云锦凤,石凤敏,刘亚玲..蒙古冰草Actin基因片段的克隆及序列分析[J].生物技术通报,2009,(7):86-89,4.基金项目
"973"项目子课题(2007CB108901-1),国家自然科学基金项目(30760159) （2007CB108901-1）
