首页|期刊导航|中国农业科学(英文版)|Analysis of RNA-Dependent RNA Polymerase Sequence of Infectious Flacherie Virus Isolated in China and Its Expression in BmN Cells
中国农业科学(英文版)2009，Vol.8Issue(7)：872-879,8.
Analysis of RNA-Dependent RNA Polymerase Sequence of Infectious Flacherie Virus Isolated in China and Its Expression in BmN Cells
Analysis of RNA-Dependent RNA Polymerase Sequence of Infectious Flacherie Virus Isolated in China and Its Expression in BmN Cells
摘要
Abstract
Full gene sequence of RNA-dependent RNA polymerase (RdRp) from Bombyx mori infectious flacherie virus isolated in Zhejiang Province, China (Zhejiang01/CHN/2002) was cloned. The sequence was 1 920 nucleotides in length coding 639 amino acid residues. Sequences comparison of RdRp showed Zhejiang01/CHN/2002 was 99.7% nucleotide sequence and 99.1% amino acids sequence homology with Japanese strain. The RdRp sequence was aligned with 8 representative picorna(-like) viruses and 8 highly conserved regions were detected. The result indicated their relevance function. Phylogenetic tree of 14 picorna(-like) viruses which RdRp presumed protein sequences revealed that the viruses from Iflavirus genus formed an independent clade. The RdRp was successfully expressed in Bran cells using Bac-to-Bac expression system.关键词
infectious flacherie virus/RNA-dependent RNA polymerase/phylogenetic analysis/BmN cells/expressionKey words
infectious flacherie virus/RNA-dependent RNA polymerase/phylogenetic analysis/BmN cells/expression分类
农业科技引用本文复制引用
LI Ming-qian,LU Qi-neng,WU Xiao-Feng,MAN Nan-a,CHEN Xiao-xue,JIN Wei,LU Xing-meng..Analysis of RNA-Dependent RNA Polymerase Sequence of Infectious Flacherie Virus Isolated in China and Its Expression in BmN Cells[J].中国农业科学(英文版),2009,8(7):872-879,8.基金项目
This work is supported by the National 863 Program of China (2006AA10A119), and the Key Project of Science and Technology Commission of Zhejiang Province, China (2003C22013). （2006AA10A119）
