第一军医大学学报2003，Vol.23Issue(9)：877-881,5.
EGFR反义RNA的转染对人类鼻咽癌CNE-2细胞EGFR的表达下调及恶性表型的抑制
Antisense epidermal growth factor receptor (EGFR) transfection down-regulates EGFR expression and suppresses the malignant phenotype of human nasopharyngeal carcinoma CNE-2 cell line
邓凡 1王瑜 2曾方银 1邹志鹏 1白晓春 1陆地 1柯志勇 1罗深秋1
作者信息
- 1. 第一军医大学细胞生物学教研室,广东,广州,510515
- 2. 福州总医院普外科,福建,福州,350025
- 折叠
摘要
Abstract
Objective To determine whether epidermal growth factor receptor (EGFR) expression contributes to tumor growth of poorly differentiated human nasopharyngeal cacinoma CNE-2 cell lines. Methods An expression vector containing a N-terminal fragment (1.35 kb) of human EGFR in the antisense orientation was transfected into CNE-2 cell lines via lipofectamine. The established clones resistant to G418 were isolated and characterized, and the tumor-inhibiting effect of antisense EGFR expression was evaluated in terms of tumor growth and metastasis at different time after subcutanenous inoculation into nude mice. Results Down-regulated EGFR expression in the cells with antisense vector transfection was demonstrated by ligand binding assay. The growth rate and the ability to grow in soft agarose of these antisense transfectants were also reduced.After inoculation into nude mice, EGFR antisense transfectants showed a longer latency period, slower tumor growth and lower metastatic rates to the lymph nodes and lung in comparison with the parental cells. Conclusions These results suggest that these EGFR antisense cDNA-transfected CNE-2 cells are of value to further delineate the role of EGFR in the development and progression ofnasopharyngeal carcinoma.关键词
表皮生长因子受体/人鼻咽癌细胞/反义RNA/基因转移Key words
epidermal growth factor receptor/human nasopharyngeal carcinoma cells/antisense RNA/gene transfer分类
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邓凡,王瑜,曾方银,邹志鹏,白晓春,陆地,柯志勇,罗深秋..EGFR反义RNA的转染对人类鼻咽癌CNE-2细胞EGFR的表达下调及恶性表型的抑制[J].第一军医大学学报,2003,23(9):877-881,5.
