首页|期刊导航|高技术通讯（英文版）|Isolation and Characterization of a New Subspecies of Aeromonas salmonicida (Aeromonas salmonicida subsp.Flounderacida subsp.Nov.) from Stone Flounder(Kareius bicoloratus L.)
高技术通讯（英文版）2004，Vol.10Issue(4)：87-93,7.
Isolation and Characterization of a New Subspecies of Aeromonas salmonicida (Aeromonas salmonicida subsp.Flounderacida subsp.Nov.) from Stone Flounder(Kareius bicoloratus L.)
Isolation and Characterization of a New Subspecies of Aeromonas salmonicida (Aeromonas salmonicida subsp.Flounderacida subsp.Nov.) from Stone Flounder(Kareius bicoloratus L.)
摘要
Abstract
Biological properties were studied to appropriate pathogenic bacteria which were isolated from di-seased (or dead) stone flounder (Kareius bicoloratus L.) which expressed bacterial septicaemia, including morphological characteristics, colony characteristics, physiological and biochemical characteristics and serum homology of isolates, the results showed that the isolates belonged to a new subspecies of A.salmonicida. In addition, the representative strains have been re-checked and detected the mol% G+C ratio of the DNA by China Center for Type Culture Collection (CCTCC), the examined strains were also regarded as a new subspecies of A.salmonicida, and designated as Aeromonas salmonicida subsp. flounderacida subsp.nov.by its isolated fish (Kareius bicoloratus). Molecular identification of analysis of the nucleotide sequence of the 16S rRNA gene were applied, the results showed high similarity (99%) with the 16S rRNA gene of Aeromonas salmonicida from GenBank database. Cluster analysis of phylogenetic tree revealed that the representative strain formed separately bootstrap-supported cluster.关键词
stone flounder (Kareius bicoloratus L.), aeromonas salmonicida subsp. flounderacida subsp.nov., biological properties, 16S rRNAKey words
stone flounder (Kareius bicoloratus L.), aeromonas salmonicida subsp. flounderacida subsp.nov., biological properties, 16S rRNA分类
信息技术与安全科学引用本文复制引用
Zhang Xiaojun(张晓君),Zhan Wenbin,Chen Cuizhen,Fang Hai..Isolation and Characterization of a New Subspecies of Aeromonas salmonicida (Aeromonas salmonicida subsp.Flounderacida subsp.Nov.) from Stone Flounder(Kareius bicoloratus L.)[J].高技术通讯（英文版）,2004,10(4):87-93,7.基金项目
This work was supported by National 863 Project (granted 2001AA628020) （granted 2001AA628020）
NSFC (granted 30271016) （granted 30271016）
Projects under the Major State Basic Research Development Program (granted G1999012002) （granted G1999012002）
Natural Science Foundation of Hebei (granted 302431). （granted 302431）
