果树学报2008，Vol.25Issue(6)：881-886,6.
香蕉束顶病毒DNA组分的克隆及其重要特征序列分析
Cloning of a DNA component of Banana bunchy top virus and analysis of its characteristic sequences
摘要
Abstract
A new full nucleotide sequence of Banana bunchy top virus(BBTV)component 6 was cloned and analyzed.The sequence contained two main characteristic sequences,the CR-SL(Stem-loop common regions)and the CR-M(Major common regions).The phylogenetic tree constructed using the amino acid sequences of BBTV 6 suggested that BBTV con mined two groups.The nucleotide and amino acid sequences of six BBTV components were aligned and compared,and the highest identities of the nucleotide and the amino acid sequences within each component were 96.10%and 98.27%respec tively.while those among the six components were 70.78%and 56.24%,respectively.The phylogenetic trees based on full nucleotide sequences and amino acid sequences of the six BBTV components indicated six groups corresponding to each BBTV component.Based on CR-M nucleotide sequence,two groups corresponding to the places of origin could be divided.In addition,the secondary structures and some physicochemical properties of proteins of the six BBTV components were pre dicted.The result showed their significant differences.关键词
香蕉束顶病毒/克隆/序列分析/特征序列Key words
Banana bunchy top virus/Clone/Sequence analysis/Characteristic sequence分类
农业科技引用本文复制引用
黄永红,梅眉,曾继吾,周碧容,吴元立,夏瑞,易干军..香蕉束顶病毒DNA组分的克隆及其重要特征序列分析[J].果树学报,2008,25(6):881-886,6.基金项目
广东省自然科学基金项目(06025368) （06025368）
