安徽农业科学2009，Vol.35Issue(19)：8848-8850,3.
甜叶悬钩子植物组织培养褐化现象控制研究
Study on the Browning Control in Tissue Culture of Rubus suavissimus S. Lee
郭治友 1钱绍方 1罗应1
作者信息
- 1. 黔南民族师范学院生命科学系,民族生物资源研究所,贵州,都匀,558000
- 折叠
摘要
Abstract
[Objective] The purpose was to seek for optimum anti-browning agent in the tissue culture of Rubus suavissimus S.Lee.[Method] With stems and leaves of R.suavissimus as explants, MS basic medium was used as the media by adding different concn.of anti-browning agent (20% Na2S2O3, Vc and AC) so as to research the effect of different anti-browning agent on tissue culture of R.suavissimus.[Result] AC and Vc had anti-browning effect on different explants.On the medium with 20% Na2S2O3, the browning extent of explants was proportional to the concn.of Na2S2O3.When the addition of 20% Na2S2O3 amounted to 20 ml, the browning of the explant was the fastest.On the medium with Vc, with the increasing addition of Vc, the medium got into a liquid or semi-solid medium and the explants died easily.On the medium with AC, when the addition of AC was 5 g/L, the browning rate of the explants could be controlled below 5%.A large number of callus could be induced on the culture medium after the anti-browning control treatment.[Conclusion]AC was the best anti-browning agent and dosage was 5 g/L.关键词
甜叶悬钩子/植物组织培养/褐化控制Key words
Rubus suavissimus S.Lee/Plant tissue culture/Brown stain control分类
农业科技引用本文复制引用
郭治友,钱绍方,罗应..甜叶悬钩子植物组织培养褐化现象控制研究[J].安徽农业科学,2009,35(19):8848-8850,3.
