安徽农业科学2009，Vol.35Issue(19)：8857-8858,8897,3.
大白菜非整倍体组织培养中污染控制研究
Preliminary Study on Contamination Control in Tissue Culture Process of Chinese Cabbage Aneuploid
摘要
Abstract
[Objective] The aim was to provide the reference for conservation of rarity genetic material of Chinese cabbage.[Method] With tissue culture seedling contaminated by bacterium and the plant in field of Chinese cabbage aneuploid as the materials and normal disinfection as CK, the tissue culture seedlings were treated by normal disinfection +different antibiotics added in culture medium, and the explants (from plant in field) were pretreated by agricultural fungicide+ normal disinfection.The controlling effects of different treatments on contamination were studied.[Result] When cultured for 7, 14 d, the contamination rates of tissue culture seedling in CK group were 9.1% and 36.4% resp..It had effective controlling effects when 100 mg/L ampicillin was added into culture medium.The contamination rate of explants in treatment of normal disinfection was 58.8% after 7 d of inoculation.Pretreatment with bactericides+ normal disinfection could effectively control contamination of bactericides, but had no obvious effect on bacterium.[Conclusion] When ampicillin was added into culture medium, explants were pretreated by optimum fungicide, the contamination in tissue culture process of Chinese cabbage aneuploid could be effectively controlled.关键词
大白菜/非整倍体/污染率Key words
Chinese cabbage/Aneuploid/Contamination rate分类
农业科技引用本文复制引用
秦垒,轩淑欣,陈雪平,王帅,申书兴..大白菜非整倍体组织培养中污染控制研究[J].安徽农业科学,2009,35(19):8857-8858,8897,3.基金项目
河北省自然科学基金项目(NO.C2005000222). （NO.C2005000222）
