安徽农业科学2009，Vol.35Issue(19)：8863-8865,3.
番茄温敏核不育系花药培养诱导愈伤组织的研究
Study on Anther Culture for Inducting Callus of Thermo-sensitive Genic Male Sterile Line of Tomato
摘要
Abstract
[Objective] The aim of the study was to investigate the interrelated factors affecting the callus induction by anther culture of thermo-sensitive genic male sterile line of tomato.[Method] This research studied the effects of different sterilization treatments, basic mediums, hormone kinds and concentrations on the anther culture for inducting callus of thermo-sensitive genic male sterile line of tomato.[Result] 0.1%HgCl2 8 min+10%NaClO 8 min was the best sterilization treatment.The contamination rate was only 1.95% and the callus induction rate achieved to 71.73%.In the comparing experiments of three kinds of basic mediums named MS, B5 and Miller, MS basic mediums had got the best effect on callus induction of the anther.The suitable concentrations of three kinds of the hormone using separately on the callus induction was 2,4-D 1 mg/L, 6-BA 2 mg/L or KT 2 mg/L.[Conclusion] The callus induction effects on the anther culture of thermo-sensitive genic male sterile line of tomato could be affected by the sterilization treatments, basic mediums, hormone kinds and concentrations.关键词
番茄/温敏核不育系/花药培养/污染率/愈伤组织诱导率Key words
Tomato/Thermo-sensitive genic male sterile line/Anther culture/Contamination rate/Callus induction rate分类
农业科技引用本文复制引用
韦鹏霄,罗丽,岑秀芬,王先裕..番茄温敏核不育系花药培养诱导愈伤组织的研究[J].安徽农业科学,2009,35(19):8863-8865,3.基金项目
国家科技部重点国际合作项目(2006DFA33380) （2006DFA33380）
广西科技厅计划项目(0718007B-38). （0718007B-38）
