安徽农业科学2009，Vol.35Issue(19)：8888-8892,5.
PCR-DGGE方法分析玉米及苜蓿青贮动态发酵体系中菌群多样性
Analysis on the Bacterial Diversity in the Dynamic Fermentation System of Corn and Alfalfa Silage by PCR-DGGE
摘要
Abstract
[Objective] The aim was to discuss the dynamic fermentation process of corn silage and alfalfa silage.[Method] With corn and alfalfa as silage materials, the genome DNA from silage microbe was extracted by benzyl chloride method.The target fragment was amplified by using primers of P2f and P3r.The dynamic fermentation diversity of corn silage and alfalfa silage were studied by PCR-DGGE.[Result] The pH value of corn silage maintained in 4.0-4.5 and alfalfa silage maintained in 5.5-6.0 during fermentation process.PCR-DGGE analysis showed that the similarity of the 2 silages were all high between 0 and 1 d, 3 and 7 d, 20 and 60 d.The 2 silages had basically similar band types.Among them, the band 1, 3, 13, 14, 17, 21 and 22 belonged to all samples and the difference was only in brightness.DGGE result showed that most sequence represented lactic-acid bacteria, and the Lactobacillus was most, followed by Lactococcus and Weissella.[Conclusion] The research laid the foundation for preparation of silage additive.关键词
PCR-DGGE/玉米青贮/苜蓿青贮/多样性Key words
PCR-DGGE/Corn silage/Alfalfa silage/Diversity分类
农业科技引用本文复制引用
韩吉雨,王海荣,侯先志,杨凯,赵子夫,郭天龙..PCR-DGGE方法分析玉米及苜蓿青贮动态发酵体系中菌群多样性[J].安徽农业科学,2009,35(19):8888-8892,5.基金项目
内蒙古科技厅草业虚拟研究院项目(20040601). （20040601）
