中国农业科学（英文版）2004，Vol.3Issue(12)：888-895,8.
Analysis of Genetic Diversity Among Sweetpotato Landraces in China
Analysis of Genetic Diversity Among Sweetpotato Landraces in China
摘要
Abstract
Genetic diversity of 48 sweetpotato landraces randomly sampled from Anhui,Fujian, Henan and Guangdong provinces in China was analyzed using RAPD, ISSR and AFLP markers. Thirty RAPD primers, 14 ISSR primers and 9 AFLP primer pairs generated 227, 249 and 260 polymorphic bands, respectively. AFLP markers were better than RAPD and ISSR markers in terms of the number of polymorphic bands detected and the experimental stability. These three molecular markers revealed the similar results that Chinese landraces exhibited a high level of genetic diversity, and the genetic variation of Guangdong landraces was significantly higher than those of the landraces from the other three regions. These results supported the hypothesis that China was a secondary center of sweetpotato diversity. The present results also supported the view that sweetpotato was first introduced to Guangdong and from there spread to other regions of China. The dendrogram based on the combined RAPD, ISSR and AFLP dataset could separate the 48 landraces into two groups: One mainly including 8 landraces from Guangdong and the other consisting of the remaining landraces from Guangdong and landraces from the other three regions. Thus, the utilization of Guangdong landraces should be specially considered in sweetpotato breeding.关键词
Sweetpotato/Landrace/Genetic diversity/RAPD/ISSR/AFLPKey words
Sweetpotato/Landrace/Genetic diversity/RAPD/ISSR/AFLP引用本文复制引用
HE Xue-qin,LIU Qing-chang,WANG Yu-ping,ZHAI Hong..Analysis of Genetic Diversity Among Sweetpotato Landraces in China[J].中国农业科学（英文版）,2004,3(12):888-895,8.基金项目
This work was partly supported by the Out standing Younger Science Foundation of China (30225028),the National High Tech R&D Program of China (863 Program,2003AA207140) and the Teaching and Research Award Program for Outstanding Young Teachers in Higher （30225028）
