军事医学科学院院刊2001，Vol.25Issue(2)：88-90,3.
SEN病毒部分基因的克隆及序列测定
Cloning and sequencing of partial gene from SENV isolate in China
詹林盛 1孟庆华 2贾帅争 1孙红琰 1杜芝燕 1王全立1
作者信息
- 1. 军事医学科学院野战输血研究所,
- 2. 北京佑安医院肝病三科,
- 折叠
摘要
Abstract
Objective:To investigate the presence of SENV infection among patients in China,and analyze partial nucleotide sequence of SENV isolated from a patient with non A-G hepatitis.Methods:A nested polymerase chain reaction (PCR) assay with primers from ORF1 of SENV genome was established to detect SENV DNA.The PCR product was cloned and sequenced.Results:SENV DNA was positive in 2 of 7 patients with non A-G hepatitis and TTV negative.Partial gene of a SENV isolate was compared with the corresponding region of SENV isolate(AX025730)from Italy and was found that the nucleotide homology was 90%.Conclusions:The results of this study confirmed the presence of SENV infection in China.The development of a PCR assay for SENV DNA detection and the cloning,sequencing of the SENV isolate have important implication for the diagnosis and epidemiological investigation on SENV infection.关键词
SEN病毒/肝炎病毒/非甲～非庚型肝炎/聚合酶链反应/序列排列分类
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詹林盛,孟庆华,贾帅争,孙红琰,杜芝燕,王全立..SEN病毒部分基因的克隆及序列测定[J].军事医学科学院院刊,2001,25(2):88-90,3.
