中国肺癌杂志2001，Vol.4Issue(2)：88-90,3.
肝素酶基因表达与肺癌转移活性的初步研究
Expression of heparanase gene and the metastatic activity of lung cancer
赵坡 1钟梅 1宋欣 1吕亚利 1王殿军 1顾峥 1陈乐真1
作者信息
摘要
Abstract
Objective To investigate the relationship between the expressionof heparanase gene and the metastatic activity of lung cancer. Methods Using the primers of heparanase gene, the expression of heparanase gene was detected by reverse transcriptase-polymerase chain reaction (RT-PCR) in two giant cell lung cancer cell lines with different metastatic activity and 56 primary tumor tissues of lung cancer and normal lung tissues. Results Both cell lines and 80％ (20／25) of primary tumor tissues of lung cancer with lymph node metastasis showed positive expression of heparanase gene, whereas 6.5％ (2／31) primary tumor tissues of lung cancer without lymph node metastasis was positive and the normal lung tissues were all negative. The positive rate for expression of heparanase gene was significantly higher in the tumor tissues with lymph node metastasis than that without lymph node metastasis (P＜0.001), higher in squamous cell carcinoma (47.2％, 17／36) than that in adenocarcinoma (20％, 4／20)(P＜0.05), and remarkably higher in the poorly differentiated tumors (65％, 13／20) than that in the well-mediate differentiated tumors (22.2％, 8／36)(P＜0.01). Conclusion The preliminary results suggest that the expression of heparanase gene may be one of the reliable markers for the metastastic activity gained by the lung cancer cells and could be used clinically in predicting the prognosis of patients.关键词
肝素酶基因/肺肿瘤/转移分类
医药卫生引用本文复制引用
赵坡,钟梅,宋欣,吕亚利,王殿军,顾峥,陈乐真..肝素酶基因表达与肺癌转移活性的初步研究[J].中国肺癌杂志,2001,4(2):88-90,3.
