中国医科大学学报2001，Vol.30Issue(2)：88-90,3.
骨髓增殖性疾病巨核系祖细胞的体外液体培养
In Vitro Liquid Culture of Megakaryocytic Progenitors in Patients With Myeloproliferative Disorders
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作者信息
- 1. 中国医科大学第一临床学院血液科,;法国Beaujon医院血液科
- 折叠
摘要
Abstract
Objective: Our purpose was to investigate if there are spontaneous megakaryocyte growth in liquid culture in vitro in patients with myeloproliferative disorders (MPD) who had spontaneous megakaryocyte colony (CFU-MK) formation. Methods: Non-adherent mononuclear cells (NMNC) from peripheral blood of the patients were cultured in serum-free medium and the expressions of c-mpl and GP-Ⅱb were examined by means of RT-PCR technique in the spontaneous growth cells. Results: There were spontaneous growth cells in serum-free liquid culture only in the patients who had spontaneous megakaryocyte colonies. A positive relation existed between them (r=0.79, P<0.01). There were parallel expressions of c-mpl and GPⅡb, which is specific to megakaryocytes, in the spontaneous growth cells. Conclusion: Megakaryocytes existed in the spontaneous growth cells in serum-free liquid culture, and derived from the same megakaryocyte progenitors as spontaneous CFU-MK, which indicated that the former would displace the spontaneous colony cells in the study on the mechanisms of spontaneous megakaryocytopoiesis in MPD.关键词
骨髓增殖性疾病/巨核细胞/液体培养分类
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..骨髓增殖性疾病巨核系祖细胞的体外液体培养[J].中国医科大学学报,2001,30(2):88-90,3.
