安徽农业科学2009，Vol.35Issue(19)：8893-8894,8900,3.
E.tenella吉林株单卵囊的分离及PCR鉴定
Isolation of E.tenella JL Strain Single-oocyst and PCR Identification
摘要
Abstract
[Objective] In order to obtain a purified strain of E.tenella, relative molecular biology researches were carried out.[Method] The single-oocyst isolation method was improved and the isolated single-oocyst which was put into capsule was fed to chickens.At the same time, the collected oocysts were identified by PCR method.[Result] The oocysts were isolated from feces of 15 chickens among that of 20 chickens and the infection rate was 75%.The PCR results demonstrated that the single-oocyst strain was E.tenella.[Conclusion] The inoculation of single-oocyst capsule was simple, besides, this method did not only save time but also declined inoculation difficulty ,increased infection rate and provided good materials for biological research of coccidia.关键词
E.tenella/单卵囊/PCR鉴定Key words
E.tenella/Single-oocyst/PCR identification分类
农业科技引用本文复制引用
杨桂连,李建华,张西臣,赵权,宫鹏涛,蔡亚南,张国才..E.tenella吉林株单卵囊的分离及PCR鉴定[J].安徽农业科学,2009,35(19):8893-8894,8900,3.基金项目
国家自然科学基金(30671580,30170696). （30671580,30170696）
