安徽农业科学2009，Vol.35Issue(19)：8909-8910,2.
Tricine-SDS-PAGE鉴定不同品质饲料蛋白质的组成
Identification of the Protein Composition of Five Different Feedstuffs with Tricine-SDS-PAGE System
摘要
Abstract
[Objective] The feasibility of the identification of protein and the analysis of the quality of different feedstuff with Tricine-SDS-PAGE system was discussed.[Method] The protein composition of five feedstuffs, rapeseed meal, cottonseed meal, extract from rapeseed meal, cottonseed meal fermented by Monilia tropicalis and cottonseed meal fermented by black mold, was identified with Tricine-SDS-PAGE system.[Results] The better isolation efficiency could be produced with 4.0%C and16.5%T.The protein band of rapeseed meal was showed in the central part(14.4-60.0 kD) and cottonseed meal, end part(about 90 kD),which contain was higher among all of experimental materials and the protein in the method was easy to be effectively separated.The result indicated that the effect of the cottonseed meal treated fermented by black mold was better that fermented by Monilia tropicalis.[Conclusion] There was significant different in protein image among five feedstuffs and the method of Tricine-SDS-PAGE ought to be the effective method in identification of the protein in feedstuff.关键词
Tricine-SDS-PAGE/饲料/蛋白质Key words
Tricine-SDS-PAGE/Feedstuff/Protein分类
农业科技引用本文复制引用
周乃继,程茂基,李爱科,潘雷..Tricine-SDS-PAGE鉴定不同品质饲料蛋白质的组成[J].安徽农业科学,2009,35(19):8909-8910,2.基金项目
国家"十一五"科技支撑计划课题(2006BAD12B04)"农副产品饲料转化和高效利用技术研究". （2006BAD12B04）
