首页|期刊导航|中国农业科学（英文版）|Cloning, Prokaryotic Expression, and Antigenicity Analysis of NS1 Gene of H9N2 Swine Influenza Virus
中国农业科学（英文版）2008，Vol.7Issue(7)：895-900,6.
Cloning, Prokaryotic Expression, and Antigenicity Analysis of NS1 Gene of H9N2 Swine Influenza Virus
Cloning, Prokaryotic Expression, and Antigenicity Analysis of NS1 Gene of H9N2 Swine Influenza Virus
摘要
Abstract
To obtain the NS1 gene of swine influenza virus H9N2 subtype expressed efficiently in E. coli, to develope the effective diagnostic methods for swine influenza virus H9N2 subtype, the NS1 gene of swine influenza virus H9N2 subtype was amplified by reverse transcriptase polymerase chain reaction (RT-PCR) and cloned into a prokaryotic expression vector, pET-28a(+), and overexpressed in E. coli BL21-DE3 after induction with 5 mmol L-1 lactose. The recombinant protein was purified by Ni-NTA and identified by western-blotting. An indirect enzyme-linked immunosorbent assay (ELISA) was used to analyze the antigenicity of the recombinant protein. The recombinant protein of NS1 was about 26 kD. The Western-blotting test showed that the recombinant protein reacted specifically with positive sera. The results of the ELISA test showed that the recombinant protein had good antigenicity.关键词
swine influenza virus, NS1, antigenicityKey words
swine influenza virus, NS1, antigenicity分类
畜牧业引用本文复制引用
WANG Fang-kun,YUAN Xiu-fang ,WANG Yi-cheng ,ZHANG Cun ,XU Li-huan ,LIU Si-dang..Cloning, Prokaryotic Expression, and Antigenicity Analysis of NS1 Gene of H9N2 Swine Influenza Virus[J].中国农业科学（英文版）,2008,7(7):895-900,6.基金项目
This study is supported by Major Program of the Zhejiang Province Commission of Science and Technology, China (2004C22044). （2004C22044）
