中国药理学通报2001，Vol.17Issue(1)：89-91,3.
牛磺酸对H2O2引起的大鼠血管平滑肌细胞核［Ca2+］
及胞浆［Ca2+］变化的影响
Effect of taurine on changes of nuclear and cytosolic ［Ca2+］
elicited by H2O2 in rat vascular smooth muscle cells
郝胜利 1张孙曦 1唐朝枢 1何其华 1凤志慧 1安丽芝1
作者信息
摘要
Abstract
AIM As one of the reactive oxygen species, toxic dose of H2O2 leads to the necrosis or apoptosis of many kinds of cells. It is nuclear whether these processes are related with the changes of nuclear Ca2+ elicited by H2O2. This study on the effect of taurine on changes of nuclear and cytosolic (Ca2+) elicited by H2O2 in rat vascular smooth muscle cells. METHODS The techniques of Fluo-3 and laser scanning confocal microscopy were used. RESULTS Rapid and sustaining rise of nuclear ［Ca2+］ (［Ca2+］n) and cytosolic ［Ca2+］(［Ca2+］c) were found in cultured rat vascular smooth muscle cells upon adding of 0.5% H2O2. Pretreatment of the cells with 20 mmol*L-1 taurine, a regulator of cellular Ca2+ homestasis, significantly decreased the rise amplitude of ［Ca2+］n (P<0.05), but failed to affect that of ［Ca2+］c (P>0.05) elicited by H2O2. It was suggested that the changes of ［Ca2+］n and ［Ca2+］c have different respond to taurine. CONCLUSION It was concluded that there exist the independent regulating mechanisms of Ca2+ in the nucleus from differences between the nucleus and cytosol Ca2+ in sensitivity to action of H2O2 and taurine.关键词
牛磺酸/H2O2/平滑肌细胞/核/共聚焦显微成像术/Fluo-3/Ca2+分类
医药卫生引用本文复制引用
郝胜利,张孙曦,唐朝枢,何其华,凤志慧,安丽芝..牛磺酸对H2O2引起的大鼠血管平滑肌细胞核［Ca2+］
及胞浆［Ca2+］变化的影响[J].中国药理学通报,2001,17(1):89-91,3.
