首页|期刊导航|南京医科大学学报（英文版）|Protection of rat islet viability following heme oxygenase-1 gene transfection via adenoviral vector in vitro
南京医科大学学报（英文版）2007，Vol.21Issue(2)：89-93,5.
Protection of rat islet viability following heme oxygenase-1 gene transfection via adenoviral vector in vitro
Protection of rat islet viability following heme oxygenase-1 gene transfection via adenoviral vector in vitro
摘要
Abstract
Objective: To investigate the effect of Heme oxygenase-1 (HO-1) gene transfection on the viability of cultured rat islets, and to explore the potential value of HO-1 gene in islet transplantation. Methods:Recombinant adenovirus vector containing human HO-1 gene(Ad-HO-1 ) or enhanced green fluorescent protein gene(Ad-EGFP) was generated by using AdEasy system respectively.The rat islets were transfected with Ad-HO-1, Ad-EGFP or blank vector and then cultured for 7 days. Transfection was confirmed by expression of EGFP and human HO-1 protein detected by fluorescence photographs and western blot, respectively. The insulin release upon different concentration of glucose stimulation was detected using insulin radioimmunoassay kit, and stimulation index (SI) was calculated. Glucose-stimulated insulin release was usedto assess islet viability. Results:Adenovirus vector successfully transferred HO-1 gene to rat islet cells in vitro, and the insulin release upon high level of glucose stimulation and stimulation index(SI) of Ad-HO-1-infected islets were significantly higher than those of Ad-EGFP-infected islets and control islets(P ＜ 0.05).Conclusion: Adenovirus-mediated HO-1 gene transfection is a feasible strategy to confer cytoprotection and therefore protect the viability of cultured rat islets.关键词
adenovirus vectors/heme oxygenase-1/pancreatic islet/gene transfectionKey words
adenovirus vectors/heme oxygenase-1/pancreatic islet/gene transfection分类
医药卫生引用本文复制引用
Xiaobo Chen,Yongxiang Li,Weiping Dong,Yang Jiao,Jianming Tan..Protection of rat islet viability following heme oxygenase-1 gene transfection via adenoviral vector in vitro[J].南京医科大学学报（英文版）,2007,21(2):89-93,5.基金项目
This work was supported by National Natural Science Foundation of China, No 30571759. （）
