安徽农业科学2009，Vol.35Issue(19)：9002-9005,4.
GnRH在青年摩杂一代水牛下丘脑-垂体-卵巢轴的免疫组化定位
Distribution of Gonadotropin (GnRH) Releasing Hormone in Hypothalamus-pituitary-ovary Axis of Youth Murrah Hybrid Water Buffalo
摘要
Abstract
[Objective] The research aimed to study the distribution of gonadotropin (GnRH) releasing hormone in hypothalamus-pituitary-ovary axis of youth murrah hybrid water buffalo.[Method] The immunohistochemistry SuperPicTureTM two step method was used to detect the distribution of GnRH in hypothalamus-pituitary-ovary axis of five femal murrah hybrid water buffalos.The association of GnRH 's distribution in hypothalamus, pituitary and ovary, and understand how the GnRH secrete releasing in hypothalamus, pituitary and ovary.[Result] The GnRH was first discovered in singulorum link of hypothalamus-pituitary-ovary axis of murrah hybrid water bufflo.The GnRH immunoreactive neurons were first discovered in every main nucleus of murrah hybrid water bufflo hypothalamus, especially distributed in supraoptic nucleus, preoptic nucleus, paraventricular nucleus most.The GnRH immunoactive productions were first discovered only in murrah hybrid water bufflo adenohypophysis for pituitary.The GnRH immunoactive productions were first discovered in the germinal epithelium cells, granulosa cells and lutein cells of murrah hybrid water bufflo.[Conclusion] The test results could provide morphological basis for the study of the GnRH's synthesis and mechanism of action, and could play reference and directions part in breeding murrah hybrid water bufflo.关键词
GnRH/下丘脑-垂体-卵巢轴/免疫组化/SuperPicTureTM二步法/摩杂一代水牛Key words
GnRH/Hypothalamus-pituitary-ovary axis/Immunohistochemistry/SuperPicTureTM two step method/Murrah hybrid water buffalo分类
农业科技引用本文复制引用
凌泽继,李瑞明,谢莹雪,周辰瑜,黎金秀,秦津,潘堂峰,宋小白,杨柄壮,许典新,韩涛,潘琼..GnRH在青年摩杂一代水牛下丘脑-垂体-卵巢轴的免疫组化定位[J].安徽农业科学,2009,35(19):9002-9005,4.基金项目
广西科学基金(桂科自0640015,桂科自0832043,桂科自0991042) （桂科自0640015,桂科自0832043,桂科自0991042）
广西教育厅科研基金(200709LX075) （200709LX075）
广西教育厅科学规划课题(C类2006C3号) （C类2006C3号）
广西大型仪器协作共用网资助. （）
