安徽农业科学2009，Vol.35Issue(19)：9006-9008,3.
冬枣花药愈伤组织的诱导与增殖
Anther Callus Induction and Propagation of Ziziphus jujuba 'Dongzao'
摘要
Abstract
[Objective] The study aimed to obtain callus from Dongzao (Ziziphus jujuba Mill.).[Method] With anthers of Z.jujuba as test materials, the effects of carbon source kinds, basic medium, and plant growth regulator on the callus induction and propagation of Z.jujuba anthers were studied.[Result]The results indicated that maltose was the optimum carbon source, MS was the optimum basic medium, and 1.0 mg/L TDZ was the optimum concentration of growth regulator for callus induction and propagation.In the medium of MS+20 g/L maltose + 1.0 mg/L TDZ, the callus induction rate was highest(86.8%).The induced callus was proliferated on MS medium supplemented with maltose 20 g/L+TDZ 1.0 mg/L.And the callus was embryogenic callus with kelly and grain-shape.关键词
冬枣/花药/愈伤组织诱导/愈伤组织增殖Key words
Ziziphus jujuba 'Dongzao'/Anther/Callus induction/Callus propagation分类
农业科技引用本文复制引用
王娜,刘孟军,秦子禹,刘晓光..冬枣花药愈伤组织的诱导与增殖[J].安徽农业科学,2009,35(19):9006-9008,3.基金项目
河北省自然科学基金项目(C2007000452). （C2007000452）
