中药材2005，Vol.28Issue(10)：900-903,4.
党参药物血清对大鼠壁细胞内游离钙离子浓度的影响
Effects of Dangshen Root Extract Serum on Intracellular Free Calcium in Gastric Parietal Cells of Dissociated Rats
摘要
Abstract
Objective: To study the effects of Dangshen root extract on intracellular free calcium concentration [ Ca2 + ] i of parietal cells. Methods: The Dangshen-containing serum was obtained from rat blood after a continuously five days' feeding with Dangshen root extract of three doses and parietal cells were isolated from Sprague-Dawley rats; [ Ca2 + ] i in single cells was measured by confocal microscope loaded with Fluo3-AM as fluorensent indicator; The change of [ Ca2+ ]i was represented by fluorensent intensity (Fl). Resuits: There were differences in the Fl of [ Ca2 + ] i increased by gastrin both between high and control group and between middle and control group ( P ＜ 0.05 ). But no difference was found between low and control groups( P ＞ 0.05). And time to peak of FI, it was not found any difference between any two of the groups. Conclusion: Dangshen-containing serum can inhibit the intracellular [ Ca2 + ] i increase induced by gastrin in a dose-dependent manner and it may be one of the mechanisms of its reduction on acid secretion which has a close relation with the formation of ulcerous diseases.关键词
党参/钙离子浓度/溃疡性疾病Key words
Dangshen Root/Free calcium concentration/Ulcerous diseases分类
医药卫生引用本文复制引用
隋峰,王汝俊,王建华,李燕舞,杜群,巫燕莉..党参药物血清对大鼠壁细胞内游离钙离子浓度的影响[J].中药材,2005,28(10):900-903,4.基金项目
国家自然科学基金资助项目(No.30271650) （No.30271650）
