中国临床康复2002，Vol.6Issue(6)：907-908,2.
恒河猴异体椎间盘移植组织学活性和生物化学研究
Study of histological viability and biochemical change of allografted intervertebral disc of rhesus monkey
周勇 1阮狄克 2陆瓞骥3
作者信息
- 1. TangDu Hospital, Fourth Military Medical University, Xi'an 710038,China
- 2. General Hospital of Navy,Beijing 100037,China
- 3. HongKong University,Hongkong
- 折叠
摘要
Abstract
Objective To investigate the histological viability and the biochemical change of fresh allografted intervertebral disc of rhesus monkey.Methods Under general anaesthesia,osteotomies of 12 rhesus monkeys were made at the endplates about 2.0 mm away from the L4～ 5 intervertibral disc,then the fresh discs were
transposed in two matched monkeys. The four groups of animals were sampled after the third, sixth,ninth and twelfth month respectively.Histological viability was assessed by 3H Proline incorporation and biochemical changes were measured by water,proteoglycan and collagen content. Results Study of histological viability demonstrated that the DPM (Disintegration Per Minute) of 3H Proline increased at the third month in anulus fibrous and the sixth month in nucleous pulposus.In the nucleus pulposus of implanted disc the study of biochemistry showed that proteoglycan content was not change obviously nine months ago, but proteoglycan content decreased at the twelfth month.Collagen contents increased at the third,sixth and ninth month. Water contents were not change among twelve months. In the annulus fibrous of implanted disc,proteoglycan and water contents decreased at the ninth month, but they were increased at the twelfth month. Collagen content increased at the sixth month,but it decreased at the ninth month again. Conclusions The present results showed the intervertibral disc grafted survived allologous transplantation.The fresh allografted intervertibral disc appeared some ability of regeneration.
关键词
intervertibral disc/allograft/uronic acid/hydroxyproline/proteoglycan,collagen/3H- Proline/water/rhesus monkey
分类
医药卫生引用本文复制引用
周勇,阮狄克,陆瓞骥..恒河猴异体椎间盘移植组织学活性和生物化学研究[J].中国临床康复,2002,6(6):907-908,2.
