生物技术通报Issue(7)：90-93,4.
高盐沉淀CTAB法提取温室菊花基因组DNA
Genomic DNA Extraction from Chrysanthemum Using High Salt CTAB Method
摘要
Abstract
Based on CTAB method,some protocal was modified according to the physiological characteristics of high containing contents of polyphenolics and polysaeeharides in Chrysanthemum.Adding 50% volume of 5 mol·L-1 NaCI into the solution which precipitating.High quality genomic DNA can be extracted from Chrysanthemum using the improved method.The DNA WaS preferable enough to be used as PCR template and digested by restriction enzyme completely and other studies of molecular biology.关键词
菊花/基因组DNA的提取/高盐沉淀CTAB法Key words
Chrysanthemum Genomic DNA extraction High salt CTAB DNA method分类
农业科技引用本文复制引用
马月萍,戴思兰..高盐沉淀CTAB法提取温室菊花基因组DNA[J].生物技术通报,2009,(7):90-93,4.基金项目
国家自然科学基金项目(30800885.30871726) （30800885.30871726）
