林业科学2009，Vol.45Issue(1)：90-96,7.
茉莉酸甲酯和日本龟蜡蚧诱导柿树挥发物对红点唇瓢虫的吸引
Attraction to the Ladybeetle by the Volatiles of Persimmon Trees Induced with Methyl Jasmonate and Japanese Wax Scale Attacking
摘要
Abstract
We studied the response ofthe ladybeetle, Chilocorus kuwanae,to volatiles released by persimmon trees(Diospyros kaki)induced either by an attack of Japanese wax scales,Ceroplastes japonicus,or by an exogenous methyl jasmonate(MeJA)appficafion.Results showed that,when C.japonicus was at the young nymph stage in July,its attack was not able to induce persimmon trees to attract ladybeetles.In the contrast,persimmon trees after sprayed with MeJA displayed a significant attraction to the ladyheetles.which showed a fluctuation rhythm ofincrease-decrease-increase again.nIe peaks of attractive responses were 78.2l%at 8 h(15:00)and 73.42%at 24 h(7:00 next morning)after the MeJA treatment.A total of 17 chemical components were detected by GC/MS from the volatiles of MeJA-treated or control persimmon trees.Among those chemicah,3 terpenoid components were higher in MeJA-treated trees than that in control trees,and 2[10]-pinene and D-limonene were detected only from the volatiles of MeJA-treated persimmon trees.These differences in the volatile compositions suggested the possible reason for the attraction of the treated persimmon trees to the ladybeefles,which was futher confirmed by the ladybeetle attraction experiment with those chemicals.Both D-limonene and α-pinene displayed a significant attractiveness(78.95%and 75.6l%.respectively)at concentration of 1×10-4 and 1×10-5g·mL-1.Therefore,it is suggested that MGA as a signal transducer may be a potential agent in regulating volatile emissions of the host plant and improving biological control of scale insects.The fluctuation in amounts of terpenoid compounds was also found in the MeJA-treated trees.Up to 8 h after treatment,the relative content of6 terpenoids all increased.but to tlIe 12 h only 0.87%α-pinene was detected.However to next 7:00 am,the 6 terpenoids were detected agoin and among them α-pinene even reached 73.01%.关键词
日本龟蜡蚧/红点唇瓢虫/柿树/茉莉酸甲酯/挥发物Key words
Ceroplastes japonicus/Chilocorus kuwanae/persimmon tree/methyl jasmonste/volatiles分类
农业科技引用本文复制引用
张艳峰,谢映平,薛皎亮,王旭..茉莉酸甲酯和日本龟蜡蚧诱导柿树挥发物对红点唇瓢虫的吸引[J].林业科学,2009,45(1):90-96,7.基金项目
This project was supported by the Grant from the National Nature Science Foendation of China(No.30471398)and the Grant from the National Nsture Science Foundation of Shanxi Province(20051066). （No.30471398）
