极地研究（英文版）2006，Vol.17Issue(2)：90-99,10.
Isolation of novel psychrophilic bacteria from Arctic sea ice
Isolation of novel psychrophilic bacteria from Arctic sea ice
摘要
Abstract
The phylogenetic diversity of culturable psychrophilic bacteria associated with sea ice from the high latitude regions of Canadian Basin and Chukchi Sea, Arctic, was investigated. A total of 34 psychropilic strains were isolated using three methods of (Ⅰ) dilution plating (at 4 ℃), (Ⅱ) bath culturing (at -1 ℃) and dilution plating, and (Ⅲ) cold shock (-20 ℃ for 24 h), bath culturing and dilution plating under aerobic conditions. Sea-ice samples were exposed to -20 ℃ for 24 h that might reduce the number of common microorganisms and encourage outgrowth of psychrophilic strains. This process might be able to be introduced to isolation psychrophilic bacteria from other environmental samples in future study. 16S rDNA nearly full-length sequence analysis revealed that psychrophilic strains felled in two phylogenetic divisions, γ-proteobacteria (in the genera Colwellia、Marinobacter、Shewanella、Glaciecola、Marinomonas and Pseudoalteromonas) and Cytophaga-Flexibacter-Bacteroides (Flavobacterium and Psychroflexus). Fifteen of bacterial isolates quite likely represented novel species (16S rDNA sequence similarity below 98%). One of strains (BSi20002) from Canadian Basin showed 100% sequence similarity to that of Marinobacter sp. ANT8277 isolated from the Antarctic Weddell sea ice, suggesting bacteria may have a bipolar distribution at the species level.关键词
Psychrophilic/Novel bacteria/Sea ice/ActicKey words
Psychrophilic/Novel bacteria/Sea ice/Actic分类
天文与地球科学引用本文复制引用
..Isolation of novel psychrophilic bacteria from Arctic sea ice[J].极地研究（英文版）,2006,17(2):90-99,10.基金项目
This study was supported by Science Foundation of China (3050000),Major Project of Chinese National Programs for Fundamental Research and Development (2004CB719601), and Marine Science Fund for Young Scientists, SOA (2005112). （3050000）
