解放军医学杂志2007，Vol.32Issue(9)：909-911,3.
1,6-二磷酸果糖对内毒素损伤性肺组织的保护作用
Protective effects of fructose-1,6-diphosphate on pulmonary tissue injured by endotoxin in rabbits
摘要
Abstract
Objective To observe the pulmonary pathologic changes of endotoxin (ET)-induced acute lung injury (ALI) in rabbits and the potential protective effects of fructose-1,6-diphosphate (FDP) on the ET-induced ALI of rabbits. Methods 24 flap-eared albation rabbits were randomly assigned to 3 groups, 8 for each, as follows: control group (group A), ET-treated group (group B) and combination group (treated by ET and FDP, group C). ALI was induced by injection of ET at one time. Group A was only injected with placebo, normal saline. ET was given to the rest groups. In group C, FDP was given as an intervening measure after rabbits injured. Rabbits were sacrificed at 6h time point. The pulmonary pathologic changes were observed. Some markers of pulmonary tissues, including the content of lipid peroxide (LPO), thromboxane B2 (TXB2), 6-keto-prostaglandin F1α(6-keto-PGF1α), interleukin-13 (IL-13) and the activity of superoxide dismutase (SOD), were observed. Results Compared with group A, the contents of LPO and TXB2 of group B showed significant increase (P＜0.05, P＜0.01), the SOD activity of group B weakened obviously (P＜0.01), the contents of 6-keto-PGF1α and IL-13 showed no statistical differences. The LPO content and the SOD activity of group C were similar to those of group A, the contents of TXB2, 6-keto-PGF1α and IL-13 of group C were much higher than those of group A (P＜0.01). Estimated by light microscope and electron microscope, the structure of lung tissue of group A is basically normal, the pathologic injuries of lung tissue of group B were much more severer and that of group C were slighter. Conclusion In the progress of ET-induced ALI, the oxidative injury, imbalance of TXB2/6-keto-PGF1α ratio and the secretion deficiency of protective cytokines play important role in inducing pathologic injuries of lung tissues. FDP can inhibit oxidative injury, ameliorate TXB2/6-keto-PGF1α balance and promote the secretion of protective cytokines, which, in turn, can protect rabbits from ET-induced ALI to some extent.关键词
果糖二磷酸盐类/呼吸窘迫综合征,成人/内毒素类/病理学/兔Key words
fructose diphosphate/respiratory distress syndrome,adult/endotoxin/pathology/rabbits分类
医药卫生引用本文复制引用
薛庆亮,汪建新,江宏,李新甫,翁翠莲..1,6-二磷酸果糖对内毒素损伤性肺组织的保护作用[J].解放军医学杂志,2007,32(9):909-911,3.基金项目
Supported by Military Medicine and Sanitation Scientific Research Foundation of the 10th Five-Year Project (01MA1114) （01MA1114）
