中国人兽共患病杂志2000，Vol.16Issue(6)：9-12,4.
抗华支睾吸虫噬菌体抗体库的构建
CONSTRUCTION OF A PHAGE DISPLAY LIBRARY OF Fab
ANTIBODIES AGAINST CLONORCHIS SINENSIS
俞慕华 1陈代雄 1王轶 1何蔼 1梁瑜 1黎宝玲 1潘恩信 2詹希美3
作者信息
- 1. 中山医科大学病原生物学部寄生虫学教研室广，510089
- 2. 广东顺德市桂洲医院内科
- 3. 广东顺德市杏坛医院卫生防疫科
- 折叠
摘要
Abstract
The total RNA extracted from PBLs of 16 patients infected with Clonorchis sinensis was reverse transcripted,and Immunoglobulin heavy chain Fd genes and light chain genes were amplified by PCR using general primers scanning γ1、γ3 Fd and κ、λ chain. After digestion with XhoI+SpeI and SacI+XbaI,the amplified Fd and light chain fragments were cloned into phagemid pComb3 and electrotransinfected competent E. coli XLl-blue respectively. The phage antibodies were displayed on the surface of recombinant phage after rescue by helper phage VCSM13. By then,a 7.5×1010clone phage Fab library against Clonorchis sinensis was constructed by using phage surface display technique.关键词
华支睾吸虫/噬菌体表面呈现/噬菌体抗体Key words
Clonorchis sinensis/Phage surface display/Phage antibody分类
医药卫生引用本文复制引用
俞慕华,陈代雄,王轶,何蔼,梁瑜,黎宝玲,潘恩信,詹希美..抗华支睾吸虫噬菌体抗体库的构建[J].中国人兽共患病杂志,2000,16(6):9-12,4.
