中国现代医学杂志2004，Vol.14Issue(24)：9-13,5.
B7.1转基因肿瘤疫苗与大蒜素联合治疗膀胱癌的初步研究
Primary study of B7-1 modified tumor vaccine combined with Allicin for bladder cancer
摘要
Abstract
Objective: This study primarily evaluated the anti-tumor effect of B7-1 modified tumor vaccine combined with Allicin for bladder tumor. Methods: A carcinogen-induced murine bladder cancer cell line, MBT-2, was transduced with lipofectin and B7-1 plasmid. The expression of B7-1 was confirmed by immunofluorescence staining. The ability of these modified cells was tested to stimulate in vitro the proliferation of syngeneic splenocytes from C3H/rHe mice. The direct cytotoxicity of Allicin in vitro was evaluated by MTT Assay. To determine whether B7-1modified tumor vaccine has a synthetic activity when it was combined with Allicin, subcutaneous bladder cancer were established in syngeneic C3H/He mice with 5×105 MBT-2 cells at the right flank. Treatments were started one week late. Mice were fed normal saline 0.1ml daily by gastric peffusion as a negative control. As in a treatment group, they were fed 2 mg Allicin daily, or injected parental MBT-2 cells or MBT-2-B7-1 cells subcutaneously at the opposite site as a vaccine, or injected MBT-2-B7-1 cells combined with Allicin gastric perfusion. Results: B7-1 modified tumor cells can stimulate splenocyte growth apparently. Combination Alliein-B7-1 gene therapy significantly inhibited the tumor growth compared with the controls, which was evident by decreased tumor incidence rate and delayed tumor growth. Conclusion: These results suggest that B7-1 modified tumor vaccine had a definite antitumor effect in a murine model of bladder cancer. Allicin treatment combined with modified tumor vaccine had significant additive anti-tumor effects. This research may provide a novel treatment modality for bladder cancer.关键词
肿瘤疫苗/大蒜素/膀胱肿瘤Key words
tumor vaccine/Allicin/bladder tumor分类
医药卫生引用本文复制引用
王坚,刘新光,何承伟,李普云,柳建军..B7.1转基因肿瘤疫苗与大蒜素联合治疗膀胱癌的初步研究[J].中国现代医学杂志,2004,14(24):9-13,5.基金项目
The Project Sponsored by the Scientific Research Foundation for the Doctor Degree of Affiliated Hospital of Guangdong Medical College (grant no. 74) and the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministr （grant no. 74）
