中国林学（英文版）2004，Vol.6Issue(3)：9-14,6.
Optimization of the uidA Gene Transfer of Rosa hybrida via Agrobacterium tumefaciens:an Assessment of Factors Influencing the Efficiency of Gene Transfer
Optimization of the uidA Gene Transfer of Rosa hybrida via Agrobacterium tumefaciens:an Assessment of Factors Influencing the Efficiency of Gene Transfer
摘要
Abstract
To develop a transformation protocol of Rosa hybrida 'Samantha' via Agrobacterium tumefaciens, the authors examined the effect of different factors on T-DNA transfer by measuring transient expression levels of an intron-containing β-glucuronidase gene. The results indicate that explant, light condition, salt concentration and acetosyringone (AS) concentration in co-culture medium are the most important factors, and factors like co-culture temperature, co-culture period and bacteria density have a strong effect on the growth of bacteria and then T-DNA transfer. Optimized co-cultivation was performed by inoculation of embryogenic callus with bacteria at a density of OD600= 0.5-0.8 for 20 min and co-culture in darkness under 23 °C on medium with 1/2 MS salts and 300 μmol·L-1 AS for 3 d.关键词
Agrobacterium tumefaciens/Rosa hybrida/uidA gene/adventitious bud/embryogenic callus/transient expressionKey words
Agrobacterium tumefaciens/Rosa hybrida/uidA gene/adventitious bud/embryogenic callus/transient expression引用本文复制引用
Gao Liping,Bao Manzhu..Optimization of the uidA Gene Transfer of Rosa hybrida via Agrobacterium tumefaciens:an Assessment of Factors Influencing the Efficiency of Gene Transfer[J].中国林学（英文版）,2004,6(3):9-14,6.基金项目
[Supported by the National Natural Science Foundation of China (Grant No.30170666)] （Grant No.30170666）
