首页|期刊导航|中国农业科学（英文版）|Construction of Genetic Linkage Map Based on SSR Markers in Peanut(Arachis hypogaea L.)
中国农业科学（英文版）2008，Vol.7Issue(8)：915-921,7.
Construction of Genetic Linkage Map Based on SSR Markers in Peanut(Arachis hypogaea L.)
Construction of Genetic Linkage Map Based on SSR Markers in Peanut(Arachis hypogaea L.)
摘要
Abstract
Molecular genetic maps of crop species can be used in a variety of ways in breeding and genomic research such as identification and mapping of genes and quantitative trait loci (QTLs) for morphological, physiological and economic traits of crop species. However, a comprehensive genetic linkage map for cultivated peanut has not yet been developed due to the extremely low frequency of DNA polymorphism in cultivated peanut. In this study, 142 recombinant inbred lines (RILs) derived from a cross between Yueyou 13 and Zhenzhuhei were used as mapping population in peanut (Arachis hypogaea L.). A total 652 pairs of genomic-SSR primer and 392 pairs of EST-SSR primer were used to detect the polymorphisms between the two parents. 141 SSR primer pairs, 127 genomic-SSR and 14 EST-SSR ones, which can be used to detect polymorphisms between the two parents, were selected to analyze the RILs population. Thus, a linkage genetic map which consists of 131 SSR loci in 20 linkage groups, with a coverage of 679 cM and an average of 6.12 cM of inter-maker distance was constructed. The putative functions of 12 EST-SSR markers located on the map were analyzed. Eleven showed homology to gene sequences deposited in GenBank. This is the first report of construction of a comprehensive genetic map with SSR markers in peanut (Arachis hypogaea L.). The map presented here will provide a genetic framework for mapping the qualitative and quantitative trait in peanut.关键词
peanut (Arachis hypogaea L.)/SSR/genetic linkage mapKey words
peanut (Arachis hypogaea L.)/SSR/genetic linkage map分类
农业科技引用本文复制引用
..Construction of Genetic Linkage Map Based on SSR Markers in Peanut(Arachis hypogaea L.)[J].中国农业科学（英文版）,2008,7(8):915-921,7.基金项目
This research was partially funded by a grant from the National Natural Science Foundation of China(30571179),and National 863 Program of China(2006AA0Z156,2006AA10A115). （30571179）
