色谱2001，Vol.19Issue(1)：91-93,3.
毛细管电泳法检测蜂蜜中残留的抗生素
Detection of Residual Antibiotics in Honey with Capillary Electrophoresis
陈天豹 1邓文汉 1卢菀华 1陈儒明 1饶平凡1
作者信息
摘要
Abstract
Five antibiotics compounds, tetracycline(TC), oxytetracycline(OTC), doxyc ycline(DOC), chlortetracycline(CTC) and chloramphenicol(CP), were successfull y separa ted and determined by high performance capillary electrophoresis(HPCE). Effects of buffer pH, various organic additives and temperature on electrophoretic separ ation of antibiotics were investigated. Satisfactory separation of these five a n tibiotics was achieved in the buffer of pH 3.2, 0.02 mol/L Na2HPO4-0.01 mol/L citric acid w ith addition of 4% (V/V) N-methylmorpholine and 12% (V/V) acetonitrile within 20 minutes. Th e calibration graphs were linear by plotting the peak area against the sample concen tration over the range of 150 μg/L to 750 μg/L and the correlat ion coeffic ients were greater than (0.991 7). The detection limits were 10 μg/L for C P, 20 μg/L for TC, OTC and DOC and 40 μg/L for CP (signal to noise ratio>5). HPCE method was successfully applied to the analysis of trace antibiotics in honey.关键词
毛细管电泳/抗生素/蜂蜜/痕量分析分类
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陈天豹,邓文汉,卢菀华,陈儒明,饶平凡..毛细管电泳法检测蜂蜜中残留的抗生素[J].色谱,2001,19(1):91-93,3.
