中国医科大学学报2001，Vol.30Issue(2)：91-93,3.
HB-EGF对系膜细胞己糖激酶活性的调节
Regulation of Hexokinase Activity by HB-EGF in Mesangial Cells
摘要
Abstract
Objective: Our aim was to study the concentrations and regulatory characteristics of heparin-binding epidermal growth factor-like factor (HB-EGF) and its functional consequences in inducing the activities of hexokinases (HKs) and net Glc utilization. Methods: The SV40MES13 cell line was used as cell model for this experiment to measure the total activity of Hks by using a standard G6PDH-coupled assay. The net Glc utilization was measured indirectly as Glc disappearance from the medium using the standard Glc oxidase-coupled reaction(Trinder). Results: The HB-EGF induced HKs activity in time- and dose-dependent manners and increased the net Glc utilization by SV40MES13 cells. Conclusion: The HB-EGF plays an important role in pathogenesis of diabetic nephropathy by inducing total HKs activity specifically and increases the net Glc utilization in mesangial cells.关键词
系膜细胞/己糖激酶/肝素结合性表皮生长因子/葡萄糖净利用分类
医药卫生引用本文复制引用
马健飞,周希静..HB-EGF对系膜细胞己糖激酶活性的调节[J].中国医科大学学报,2001,30(2):91-93,3.
